(16) In darkness, raising the concentration of K in the fluid of perfusion gives an increase of the efflux of (86)Rb and increasing the extracellular concentration of Ca yields a retention.(15) This suggests that many retinal ganglion cells continue to discharge in total darkness for long periods. (14) In lettuce, the presence of 2,4-D in the light lowered the concentration of total Hg (or MeHg) required to reduce growth by 50%, about 13 times relative to that in the dark (i.e., it sensitized the cells).(13) The extracellular concentration of GABA is probably high in prolonged darkness, and it is low after prolonged light exposure.(12) Although the Ca2+-independent mechanism accounts for about two thirds of the total acetylcholine release in the dark, the amount of acetylcholine released in this way is small compared with the release of acetylcholine triggered by stimulation of the retina with light.(11) The results indicate that CRALBP X 11-cis-retinol is sufficiently stereoselective in its binding properties to warrant consideration as a component of the mechanism for the generation of 11-cis-retinaldehyde in the dark.(10) The second triplet, which was stable in the dark at 4.2 K following illumination, was assigned to the radical pair Donor+I.(9) Steady state levels of chloroplast mRNA encoding the core PSII polypeptides remain nearly constant in the light or the dark and are not affected by the developmental stage of the plastid.(8) Previous FTIR measurements have identified several tyrosine residues that change their absorption characteristics between light-adapted BR and dark-adapted BR, or between intermediates K and M [Dollinger, G., Eisenstein, L., Lin, S.-L., Nakanishi, K., Odashima, K., & Termini, J.(7) We were searching for spontaneous and positional nystagmus in 5 positions with open eyes in darkness and with closed eyes.(6) Urinalysis revealed a low pH, increased ketones and bilirubin excretion, dark yellowish change in color, the appearance of "leaflet-shaped" crystals and increased red blood cells and epithelial cells in the urinary sediment, increased water intake, decreased specific gravity and decreased sodium, potassium and chloride in the urine.(5) In the dark cortical zone of the nodes (III group) there occur tissue basophils (mast cells), that, together with increasing number of acidophilic granulocytes and appearance of neutrophilic cells, demonstrates that there is an inflammatory reaction in the organ studied as a response to the lymphocytic suspension injected.(4) Most notably, retroperitoneal lymph nodes in rabbits remained dark blue up to 28 days after hindlimb endolymphatic instillation of liposomal patent blue.(3) In the dark the 6-azidoflavoproteins are quite stable, except for L-lactate oxidase, where spontaneous conversion to the 6-amino-FMN enzyme occurs slowly at pH 7.(2) The first group was reared in complete darkness while the second one was subjected to permanent noise.(1) And that, as much as the “on water, operational” considerations, is why we are being kept in the dark.
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